Alternative Names:

APAF-3; Caspase9; ICE-LAP6; Apoptotic protease Mch-6; CASP-9; Caspase-9; Caspase-9 precursor; MCH6; RNCASP9;

Application:

WB IHC ICC/IF FC IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Caspase-9 in HeLa cell lysate treated with Camptothecin.
	Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody.
	Immunofluorescent analysis of 3T3 cells, using Caspase-9 Antibody.

Purification:

Affinity-chromatography

Specificity:

Caspase-9 Antibody detects endogenous levels of total Caspase-9

Immunogen:

A synthesized peptide derived from human Caspase-9

Description:

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.