Alternative Names:

eplication protein C; PP2A-alpha; Replication protein C; RP-C; PPP2CA; MGC786

Application:

WB IHC ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Phospho-PP2A alpha (Y307) in (1) Rat kidney lysate; (2) A431 cell lysate treated with EGF.

Purification:

Affinity-chromatography

Specificity:

Phospho-PP2A alpha (Y307) Antibody detects endogenous levels of total Phospho-PP2A alpha (Y307)

Immunogen:

A synthesized peptide derived from human Phospho-PP2A alpha (Y307)

Description:

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.