Alternative Names:

RING finger protein 20; BRE1A;RNF20;

Application:

WB ICC/IF IP FC

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

	Western blot analysis of RNF20 in (1) MCF7 cell lysate; (2) HeLa cell lysate.

Purification:

Affinity-chromatography

Specificity:

RNF20 Antibody detects endogenous levels of total RNF20

Immunogen:

A synthesized peptide derived from human RNF20

Description:

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.