Alternative Names:

Estrogen-induced tag 6; HPH-3;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

	Western blot analysis of PHD1 in (1) HeLa cell lysate; (2) A549 cell lysate.

Purification:

Affinity-chromatography

Specificity:

PHD1/prolyl hydroxylase Antibody detects endogenous levels of total PHD1/prolyl hydroxylase

Immunogen:

A synthesized peptide derived from human PHD1/prolyl hydroxylase

Description:

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.