Alternative Names:

DNA binding protein SATB1; DNA-binding protein SATB1; SATB homeobox 1;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50~1:100 FC 1:200~1:500

	Western blot analysis of SATB1 in (1)Mouse thymus tissue lysate;(2)Jurkat cell lysate.
	Immunohistochemical analysis of paraffin-embedded human thymus, using SATB1 Antibody.

Purification:

Affinity-chromatography

Specificity:

SATB1 Antibody detects endogenous levels of total SATB1

Immunogen:

A synthesized peptide derived from human SATB1

Description:

SATB1 is a crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.