Alternative Names:

Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

Application:

WB IHC ICC/IF

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of FoxO3a in (1) Jurkat cell lysate; (2) SH-SY5Y cell lysate.
	Immunohistochemical analysis of paraffin-embedded human bladder carcinoma, using FoxO3a Antibody.
	Immunofluorescent analysis of Hela cells, using FoxO3a Antibody .

Purification:

Affinity-chromatography

Specificity:

FoxO3a Antibody detects endogenous levels of total FoxO3a

Immunogen:

A synthesized peptide derived from human FoxO3a

Description:

FoxO3a is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.