Alternative Names:

CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; P21cip1;

Application:

WB IHC ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) LnCaP cell lysate.
	Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody.
	Immunofluorescent analysis of MCF7 cells, using p21 Antibody .

Purification:

Affinity-chromatography

Specificity:

p21 Antibody detects endogenous levels of total p21

Immunogen:

A synthesized peptide derived from human p21

Description:

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.