Alternative Names:

NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

Application:

WB FC IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 FC 1:50 IP 1:50

	Western blot analysis of Phospho-NF-κB p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha.

Purification:

Affinity-chromatography

Specificity:

Phospho-NF-κB p65 (S529) Antibody detects endogenous levels of total Phospho-NF-κB p65 (S529)

Immunogen:

A synthesized peptide derived from human NF-κB p65

Description:

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.