Alternative Names:

H3 histone family, member A; H3/A; H31; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A

Application:

WB IHC ICC/IF FC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC 1:50~1:200 FC 1:50

	Western blot analysis of Phospho-Histone H3 (Thr3) in HeLa cell lysates treated with FBS + Calyculin A.
	Immunohistochemical analysis of paraffin-embedded human tonsil, using Phospho-Histone H3 (T3) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-Histone H3 (T3) Antibody detects endogenous levels of total Phospho-Histone H3 (T3)

Immunogen:

A synthesized peptide derived from human Phospho-Histone H3 (T3)

Description:

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.