Alternative Names:

hSmad5; JV5-1 ; MADH5; SMAD5; MusMLP ;SMAD family member 5;

Application:

WB IHC ICC/IF

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of Phospho-Smad5 in (1)Mouse brain tissue lysate; (2)Rat brain tissue lysate.
	Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-Smad5 (S463/465) Antibody detects endogenous levels of total Phospho-Smad5 (S463/465)

Immunogen:

A synthesized peptide derived from human Phospho-Smad5 (S463/465)

Description:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.