Alternative Names:

JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

Application:

WB IHC ICC/IF

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of Phospho-Smad3(S423/S425) expression in PC-12 cell lysate treated with 1%BSA.
	Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.
	Immunofluorescent analysis of A549 cells treated with TGFβ , using Phospho-Smad3 (S423 + S425) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-Smad3 (S423 + S425) Antibody detects endogenous levels of total Phospho-Smad3 (S423 + S425)

Immunogen:

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)

Description:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.