Alternative Names:

GRP-78; GRP78; BIP; MIF2; HSPA5

Application:

WB IHC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200

	Western blot analysis of GRP78 BiP expression in (1) HeLa cell lysate; (2) C6 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human testis, using GRP78 BiP Antibody.

Purification:

Affinity-chromatography

Specificity:

GRP78 BiP Antibody detects endogenous levels of total GRP78 BiP

Immunogen:

A synthesized peptide derived from human GRP78 BiP

Description:

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.