Alternative Names:

DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK

Application:

WB IHC ICC/IF

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate.
	Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-DNA PKcs (S2056) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-DNA PKcs (S2056) Antibody detects endogenous levels of total Phospho-DNA PKcs (S2056)

Immunogen:

A synthesized peptide derived from human Phospho-DNA PKcs (S2056)

Description:

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.