Alternative Names:

A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein P1H12; Gicerin; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

	Western blot analysis of CD146 expression in (1) A375 cell lysate;(2) HUVEC cell lysate.
	Immunohistochemical analysis of paraffin-embedded human uterus, using CD146 Antibody.
	Immunofluorescent analysis of A375 cells, using CD146 Antibody.

Purification:

Affinity-chromatography

Specificity:

CD146 Antibody detects endogenous levels of total CD146

Immunogen:

A synthesized peptide derived from human CD146

Description:

CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.