Alternative Names:

C-erbB-2; ErbB2; HER2, MLN 19; NEU; NEU proto-oncogene; NGL; Receptor protein-tyrosine kinase erbB-2;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

	Western blot analysis of ErbB2 (HER2) expression in SKBR-3 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ErbB2 (HER2) Antibody.
	Immunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody.

Purification:

Affinity-chromatography

Specificity:

ErbB2 (HER2) Antibody detects endogenous levels of total ErbB (HER2)

Immunogen:

A synthesized peptide derived from human ErbB2 (HER2)

Description:

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.