Alternative Names:

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

Application:

WB IHC ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Phospho-p53 (Ser392) expression in HEK293 whole cell lysate.
	Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-p53 (S392) Antibody detects endogenous levels of total Phospho-p53 (S392)

Immunogen:

A synthesized peptide derived from human Phospho-p53 (S392)

Description:

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.