Alternative Names:

Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; CRK; CRKII; Proto-oncogene c-Crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian)

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis of CrkII expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

CrkII Antibody detects endogenous levels of total CrkII

Immunogen:

A synthesized peptide derived from human CrkII

Description:

Crk an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.