Alternative Names:

Apopain precursor; CASP-3; CPP-32; CPP32; Caspase-3; Cysteine protease CPP32; ICE3; SCA-1; SREBP cleavage activity 1; Yama protein,p17

Application:

WB IHC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of extracts from COLO cells, using Caspase 3 (p17,Cleaved-Asp175) Antibody. The lane on the left is treated with the synthesized peptide.

Purification:

Affinity-chromatography

Specificity:

Caspase 3 (p17,Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

Immunogen:

The antiserum was produced against synthesized peptide derived from human Caspase 3.

Description:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.