Alternative Names:

ADP-ribosyltransferase 1; ADPRT; ADPRT1; pADPRT-1; PARP; PARP-1; poly (ADP-ribose) pol; poly [ADP-ribose] polymerase 1; poly[ADP-ribose] synthetase 1; PPOL

Application:

WB

Reactivity:

Human,Mouse

Dilution:

WB 1:500-1:2000

	Western blot analysis of extracts from A549 cells,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

PARP (Cleaved-Asp214) Antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214.

Immunogen:

The antiserum was produced against synthesized peptide derived from human PARP.

Description:

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.