Alternative Names:

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphoinositide-3-kinase catalytic alpha polypeptide; PI3-kinase p110 alpha; PI3K; PI3K p110-alpha; PK3CA; PtdIns-3-kinase p110

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:1000~1:2000 ICC/IF 1:50~1:200 IP 1:20

	Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.

Purification:

Affinity-chromatography

Specificity:

PI 3 Kinase catalytic subunit alpha detects endogenous levels of total PI 3 Kinase catalytic subunit alpha

Immunogen:

A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha

Description:

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.