Alternative Names:

UCH-L3; Ubiquitin thiolesterase L3; Ubiquitin carboxyl-terminal hydrolase isozyme L3;UCHL3;

Application:

WB

Reactivity:

Human Mouse Rat

Dilution:

WB 1:1000~1:2000

	Western blot analysis of UCHL3 expression in 293T cell lysate.

Purification:

Affinity-chromatography

Specificity:

UCHL3 Antibody detects endogenous levels of total UCHL3

Immunogen:

A synthesized peptide derived from human UCHL3

Description:

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.