Alternative Names:

hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; HR21;KIAA0078; NXP1;Rad21;

Application:

WB FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 FC 1:50

	Western blot analysis of Rad21 expression in HeLa cell lysate.

Purification:

Affinity-chromatography

Specificity:

Rad21 Antibody detects endogenous levels of total Rad21

Immunogen:

A synthesized peptide derived from human Rad21

Description:

Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspaselike enzyme, allows chromosomal separation. Rad21 interacts with Rec8 to form a cohesin complex that functions in sister chromatid alignment. Rad21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.