Alternative Names:

CD20; CK-20; CK20; cytokeratin 20; Cytokeratin-20; K1C20; K20; keratin 20; Keratin, type I cytoskeletal 20; Keratin-20; KRT20; KRT21; MGC35423; Protein IT;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:50

	Western blot analysis of Cytokeratin 20 expression in SW480 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human colon, using Cytokeratin 20 Antibody.
	Immunofluorescent analysis of Hela cells, using Cytokeratin 20 Antibody .

Purification:

Affinity-chromatography

Specificity:

Cytokeratin 20 Antibody detects endogenous levels of total Cytokeratin 20

Immunogen:

A synthesized peptide derived from human Cytokeratin 20

Description:

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract. Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.