Alternative Names:

ADPRT; ADP-Ribosyltransferase; CPP32; PARP; PARP1; SCA1; PPOL; YAMA;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

	Western blot analysis of PARP expression in Jurkat cell lysate.
	Immunohistochemical analysis of paraffin-embedded human testis, using PARP Antibody.
	Immunofluorescent analysis of Hela cells, using PARP Antibody .

Purification:

Affinity-chromatography

Specificity:

PARP Antibody detects endogenous levels of total PARP

Immunogen:

A synthesized peptide derived from human PARP

Description:

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.