Alternative Names:

Luciferase; Firefly; Luciferin 4 monooxygenase;

Application:

WB ICC/IF

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200

	Western blot analysis of Firefly Luciferase Antibody - N-terminal expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
	Immunofluorescent analysis of 293 cells transfected with Firefly Luciferase, using Firefly Luciferase Antibody .

Purification:

Affinity-chromatography

Specificity:

Firefly Luciferase Antibody detects endogenous levels of total Firefly Luciferase

Immunogen:

A synthesized peptide derived from Firefly Luciferase

Description:

Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.