Alternative Names:

Renilla-type luciferase; Renilla luciferin 2 monooxygenase;

Application:

WB ICC/IF

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200

	Western blot analysis of Renilla Luciferase expression in (1) Renilla Luciferase transfected 293 cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
	Immunofluorescent analysis of 293 cells transfected with Renilla Luciferase, using Renilla Luciferase Antibody .

Purification:

Affinity-chromatography

Specificity:

Renilla Luciferase Antibody detects endogenous levels of total Renilla Luciferase

Immunogen:

A synthesized peptide derived from Renilla Luciferase

Description:

Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + light. The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.