Alternative Names:

Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:100 IP 1:50 FC 1:50

	Western blot analysis of Alkaline Phosphatase expression in (1)HepG2 cell lysate; (2)JAR cell lysate.
	Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
	Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .

Purification:

Affinity-chromatography

Specificity:

Alkaline Phosphatase Antibody detects endogenous levels of total Alkaline Phosphatase

Immunogen:

A synthesized peptide derived from human Alkaline Phosphatase

Description:

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.