Alternative Names:

HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;HistoneH3/j ;Histone H3/k; Histone H3/l;HIST3H3;

Application:

WB IHC ICC/IF

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:500~1:2000 ICC/IF 1:100~1:500

	Western blot analysis of Histone H3 (mono methyl K18) expression in HeLa cell lysate.
	Immunohistochemical analysis of paraffin-embedded human liver, using Histone H3 (mono methyl K18) Antibody.

Purification:

Affinity-chromatography

Specificity:

Histone H3 (mono methyl K18) Antibody detects endogenous levels of total Histone H3 (mono methyl K18)

Immunogen:

A synthesized peptide derived from human Histone H3 (mono methyl K18)

Description:

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.