Alternative Names:

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500~1:2000

	Western blot analysis of Phospho-p53 (S376) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid.

Purification:

Affinity-chromatography

Specificity:

Phospho-p53 (S376) Antibody detects endogenous levels of total Phospho-p53 (S376)

Immunogen:

A synthesized peptide derived from human Phospho-p53 (S376)

Description:

Plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.