Alternative Names:

ARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:500~1:1000 ICC/IF 1:100~1:500 FC 1:200

	Western blot analysis of Rho A + B + C expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody .
	Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .

Purification:

Affinity-chromatography

Specificity:

Rho A + B + C Antibody detects endogenous levels of total Rho A + B + C

Immunogen:

A synthesized peptide derived from human Rho A + B + C

Description:

RhoA , RhoB , RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.