Alternative Names:

CBX5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1: 50

	Western blot analysis of HP1 alpha expression in SH-SY5Y cell lysate.

Purification:

Affinity-chromatography

Specificity:

HP1 alpha Antibody detects endogenous levels of total HP1 alpha

Immunogen:

A synthesized peptide derived from human HP1 alpha

Description:

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.