Alternative Names:

DGCR8; DGCRK6; Gy1 ; C22orf12; D16Wis2; pasha;

Application:

WB ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 ICC/IF 1:100~1:500 IP 1:50

	Western blot analysis of DGCR8 expression in (1) HEK293 cell lysate; (2) HeLa cell lysate.

Purification:

Affinity-chromatography

Specificity:

DGCR8 Antibody detects endogenous levels of total DGCR8

Immunogen:

A synthesized peptide derived from human DGCR8

Description:

Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.