Alternative Names:

Oncogene PIM1; PIM; pim-1 kinase 44 kDa isoform; pim-1 oncogene; pim-1 oncogene (proviral integration site 1); PIM1;

Application:

WB IHC ICC/IF

Reactivity:

Human Mouse

Dilution:

WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of PIM1 expression in Jurkat cell lysate.
	Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PIM1 Antibody .

Purification:

Affinity-chromatography

Specificity:

PIM1 Antibody detects endogenous levels of total PIM1

Immunogen:

A synthesized peptide derived from human PIM1

Description:

Pim1 a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.