Alternative Names:

ACL; ATPCL; CLATP; ATP citrate lyase;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of ATP citrate lyase expression in HeLa cell lysate.
	Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody .

Purification:

Affinity-chromatography

Specificity:

ATP citrate lyase Antibody detects endogenous levels of total ATP citrate

Immunogen:

A synthesized peptide derived from human ATP citrate lyase

Description:

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.