Alternative Names:

PKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of AKT1/2/3 expression in A549 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human testis, using AKT1/2/3 Antibody.
	Immunofluorescent analysis of MCF-7 cells, using AKT1/2/3 Antibody.

Purification:

Affinity-chromatography

Specificity:

AKT1/2/3 Antibody detects endogenous levels of total AKT1/2/3

Immunogen:

A synthesized peptide derived from human AKT1/2/3

Description:

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.