Alternative Names:

Early endosome antigen 1; EEA1; MST105; ZFYVE2;

Application:

WB ICC/IF

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200

	Western blot analysis of EEA1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Purification:

Affinity-chromatography

Specificity:

EEA1 Antibody detects endogenous levels of total EEA1

Immunogen:

A synthesized peptide derived from human EEA1

Description:

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.