Alternative Names:

Signal transducer and activator of transcription 5A; STAT5; STAT5A; STAT5B; Transcription factor STAT5A; Transcription factor STAT5B; MGF;

Application:

WB IHC

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200

	Western blot analysis of Phospho-Stat5 (Y694) expression in A431 cell lysate treated with EGF.
	Immunohistochemical analysis of paraffin-embedded human thyroid, using Phospho-Stat5 (Y694) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-Stat5 (Y694) Antibody detects endogenous levels of total Phospho-Stat5 (Y694)

Immunogen:

A synthesized peptide derived from human Phospho-Stat5 (Y694)

Description:

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation. This phosphorylation is mediated by Src upon erythropoietin stimulation. Stat5 is constitutively active in some leukemic cell types.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.