Alternative Names:

I-kappa-B-alpha; IKBA; IkappaBalpha; MAD3; NFKBI; NFKBIA; RL/IF-1;

Application:

WB IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IP 1:50

	Western blot analysis of Phospho-IKB alpha (S32) expression in HeLa cell lysate treated with Calyculin A and TNF-a.

Purification:

Affinity-chromatography

Specificity:

Phospho-IKB alpha (S32) Antibody detects endogenous levels of total Phospho-IKB alpha (S32)

Immunogen:

A synthesized peptide derived from human Phospho-IKB alpha (S32)

Description:

Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.