Alternative Names:

EC 2.7.10.1; ERBB1; Epidermal growth factor receptor precursor; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR;

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Phospho-EGFR (Y1086) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with EGF.

Purification:

Affinity-chromatography

Specificity:

Phospho-EGFR (Y1086) Antibody detects endogenous levels of Phospho-EGFR (Y1086)

Immunogen:

A synthesized peptide derived from human Phospho-EGFR (Y1086)

Description:

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phospho-EGF Receptor (Tyr1086) Antibody is directed against a previously unpublished EGF receptor phosphorylation site at Tyr1086, Phosphorlation of EGF receptor at Tyr1086 was observed in select carcinoma cell lines and in tumors.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.