Alternative Names:

RPTOR; KOG1; KIAA1303; RAPTOR; Mip1;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IHC 1:50~1:100 ICC/IF 1:50~1:100 IP 1:30 FC 1:30

	Western blot analysis of Raptor expression in MCF-7 cell lysate.

Purification:

Affinity-chromatography

Specificity:

Raptor Antibody detects endogenous levels of Raptor

Immunogen:

A synthesized peptide derived from human Raptor

Description:

The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets. Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates. Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling. This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.