Alternative Names:

ERN1, ER to nucleus signalling 1, Inositol-requiring enzyme 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, HIRE1p, IRE1, Inositol-requiring 1, IRE1P;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500~1:2000

	Western blot analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.

Purification:

Affinity-chromatography

Specificity:

Phospho-IRE1 (S724) Antibody detects endogenous levels of Phospho-IRE1 (S724)

Immunogen:

A synthesized peptide derived from human Phospho-IRE1 (S724)

Description:

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.