Alternative Names:

REPA2; RF-A; RP-A; RPA2; RPA32; replication factor-A protein 2; replication protein A 32 kDa subunit; replication protein A2;

Application:

WB IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:5000~1:10000 IP 1:20

	Western blot analysis of Phospho-RPA2 (T21) expression in HeLa cell lysate treated with Calyculin A.

Purification:

Affinity-chromatography

Specificity:

Phospho-RPA2 (T21) Antibody detects endogenous levels of Phospho-RPA2 (T21)

Immunogen:

A synthesized peptide derived from human Phospho-RPA2 (T21)

Description:

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.