Alternative Names:

SB1.8/DXS423E protein; SM1A; SMC1A; SMC1L1; SMC1alpha protein; Sb1.8;

Application:

WB IHC ICC/IF

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of Phospho-SMC1 (S957) expression in (1) HeLa cell lysate; (2) Hela cells lysate treated with AP.
	Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-SMC1 (S957) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-SMC1 (S957) Antibody detects endogenous levels of Phospho-SMC1 (S957)

Immunogen:

A synthesized peptide derived from human Phospho-SMC1 (S957)

Description:

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair . ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint. SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.