Alternative Names:

Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; CRK; CRKII; Proto-oncogene c-Crk;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000~1:2000 ICC/IF 1:50~1:200

	Western blot analysis of Phospho-CrkII (Tyr221) expression in (1) K562 cell lysate treated with AP; (2) K562 cell lysate.

Purification:

Affinity-chromatography

Specificity:

Phospho-CrkII (Tyr221) Antibody detects endogenous levels of Phospho-CrkII (Tyr221)

Immunogen:

A synthesized peptide derived from human Phospho-CrkII (Tyr221)

Description:

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.