Alternative Names:

Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog (S. pombe); Serine/threonine-protein kinase Chk1;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500~1:2000

	Western blot analysis of Phospho-Chk1 (S296) expression in HEK293 cell lysate Treated with Calyculin.

Purification:

Affinity-chromatography

Specificity:

Phospho-Chk1 (S296) Antibody detects endogenous levels of Phospho-Chk1 (S296)

Immunogen:

A synthesized peptide derived from human Phospho-Chk1 (S296)

Description:

Chk1 kinase acts downstream of ATM/ATR kinase and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.