Alternative Names:

NMP200; nuclear matrix protein 200; pre-mRNA-processing factor 19; PRP19; PRP19/PSO4; PRPF19; PSO4; senescence evasion factor; SNEV; UBOX4;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

	Western blot analysis of PRP19 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human cervix cancer, using PRP19 Antibody.

Purification:

Affinity-chromatography

Specificity:

PRP19 Antibody detects endogenous levels of PRP19

Immunogen:

A synthesized peptide derived from human PRP19

Description:

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.