Alternative Names:

MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; CASP9; Caspase-9; Caspase 9 Dominant Negative;

Application:

WB IP

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 IP 1:50

	Western blot analysis of cleaved Caspase-9 Antibody expression in HeLa cell lysate treated with staurosporine.

Purification:

Affinity-chromatography

Specificity:

cleaved Caspase-9 Antibody detects endogenous levels of cleaved Caspase-9

Immunogen:

A synthesized peptide derived from human cleaved Caspase-9

Description:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.