Alternative Names:

28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27;

Application:

WB IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IP 1:50

	Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock.

Purification:

Affinity-chromatography

Specificity:

Phospho-Hsp27 (S82) Antibody detects endogenous levels of Phospho-Hsp27 (S82)

Immunogen:

A synthesized peptide derived from human Phospho-Hsp27 (S82)

Description:

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.