Alternative Names:

CD14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein;

Application:

WB IHC ICC/IF

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

	Western blot analysis of CD14 expression in Human tonsil cell lysate.
	Immunohistochemical analysis of paraffin-embedded human breast, using CD14 Antibody.
	Immunofluorescent analysis of K562 cells, using CD14 Antibody.

Purification:

Affinity-chromatography

Specificity:

CD14 Antibody detects endogenous levels of CD14

Immunogen:

A synthesized peptide derived from human CD14

Description:

CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.