Alternative Names:

Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:500~1:1000 ICC 1:50~1:200

	Western blot analysis of Phospho-Smad5 (S463/S465) expression in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate.

Purification:

Affinity-chromatography

Specificity:

Phospho-Smad5 (S463/S465) Antibody detects endogenous levels of Phospho-Smad5 (S463/S465)

Immunogen:

A synthesized peptide derived from human Smad5

Description:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.